Drosophila larval NMJ immunohistochemistry.

نویسندگان

  • Jonathan Brent
  • Kristen Werner
  • Brian D McCabe
چکیده

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use of the Drosophila motor system is due to its high accessibility. It can be analyzed with single-cell resolution. There are 30 muscles per hemisegment whose arrangement within the peripheral body wall are known. A total of 31 motor neurons attach to these muscles in a pattern that has high fidelity. Using molecular biology and genetics, one can create transgenic animals or mutants. Then, one can study the developmental consequences on the morphology and function of the NMJ. Immunohistochemistry can be used to clearly image the components of the NMJ. In this article, we demonstrate how to use antibody staining to visualize the Drosophila larval NMJ.

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عنوان ژورنال:
  • Journal of visualized experiments : JoVE

دوره 25  شماره 

صفحات  -

تاریخ انتشار 2009